Abstract
The K. F. of the acid-fast bacteria was the strongest in the period where they were stainded strongly by Ziehl and their growth was vigorous. It was also stronger when we had double no staining. The pH of the boiling water influenced on the K. F., that is, strongest in neutral, and weaker in acid or alkaline. Among the neutral waters, it is stronger with destilled water than with pipe-water or physiologicol salt solution. Therefore, destilled water seems the best to examine the K. F.
