鼠癩化学療法剤のScreening testに関する基礎的実験(其の3)

余等の皮下接種法とChang氏腹腔内接種法の比較実験並に討論

抄録

This basic study was conducted in order to introduce the subcutaneous inoculation method devised by the authors and Chang's intraperitoneal method. Various therapeutic agents were tested by the two methods. The screening tests by intravenous, corneal, subdural, intraperitoneal and other inoculation methods used by other investigators are discussed. The value of the method of subcutaneous inoculation of dilute emulsion of the authors and the method of intraperitoneal inoculation of concentrated emulsion of Chang is discussed from the standpoint of time relation, technique, trustworthiness, quantitative relationship and reproducibility.
The observations are summarized here.
1) The time required for evaluation is the same with either method but by using a 10-3 emulsion in the subcutaneous inoculation method, it is possible to shorten the time.
2) With the subcutaneous inoculation method, evaluation is limited to a single point, the site of inoculation, and as measurement can be made accurately with a gauge, the technique is simple and accurate.
3) The subcutaneous inoculation method revealed the limits of the onset inhibiting action of known agents as INAH, SM and DDS with both the dilute and concentrated emulsions. It is believed that this finding is sufficient evidence for trussing the accuracy of the evaluations.
4) For screening very small quantities of synthetic or extracted samples, subcutaneous inoculation of 0.2 ml of 10-4 emulsion is more appropriate than the intraperitoneal inoculation method.
5) Reproducibility is more accurate with the subcutaneous inoculation method which is technically simpler and evaluation is more objective and accurate compared with the intraperitoneal inoculation method.