らい菌の精製,特に精製らい菌ワクチンの宿主マクロファージ細胞機能に及ぼす効果について

斎藤 肇; 冨岡 治明; 佐藤 勝昌

doi:10.5025/hansen1977.56.101

斎藤肇, 冨岡治明, 佐藤勝昌

著者情報

ジャーナルフリー

1987 年 56 巻 3 号 p. 101-109

DOI https://doi.org/10.5025/hansen1977.56.101

詳細

発行日: 1987/09/30 受付日: 1987/07/16 J-STAGE公開日: 2008/02/26 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/26 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : M. leprae purified by the method of Draper (IMMLEP Protocol 1/79) based on Percoll gra-dient centrifugation and aqueous two phase separation system and the method of Mori et al. based on the Percoll gradient ultracentrifugation were compared for some of their biological activities. The integrity of purified leprosy bacilli was higher in the latter preparation than the former. When the vaccines prepared from M. leprae cells obtained by the two purifica-tion methods by heat-treatment at 100°C were studied for their efficacy to modulate host macro-phage cell functions, the two vaccines were able to potentiate the phagocytic and active oxy-gen generating abilities but unable to augment the antilistierial activity of the host macropha-ges by double injections 21 days and 4 days before harvest via subcutaneous and intraperitoneal routes, respectively. M. leprae vaccine obtained by Draper's method more markedly height-ened the phagocytic activity of host macrophages than that prepared by Mori's meth d, where-as the inverse was the case for enhancement of the active oxygen producing ability of the host macrophages.
There are at least three approaches for preparing antileprosy vaccines: mycobacteria other than Mycobacterium leprae, which share antigenic determinants with M. leprae (^{1, 2}); M. leprae derived from tissues of infected animals such as armadillos(³); and M. leprae specificantigenic proteins produced in certain microbes, such as Escherichia coli, to which the cloned DNA of M. leprae was transferred(^{4, 5}). Although some recombinant proteins of M. leprae have been reported to be useful for clinical diagnosis(^4-6), their efficacy as a protective antigen against M. leprae infection remains to be confirmed. Convit et al.(⁷) reported that a combination of killed M. leprae with live BCG was markedly efficacious as a vaccine to control borderline and lepromatous leprosy. Shepard et al.(⁸) also reported the efficacy of the same vaccine against M. leprae infection in mice. For preparation of M. leprae vaccine, the establishment of a method to obtain active M. leprae cells with a high purity and in high yield are necessary. In this study, we compared two purification methods for M. leprae, Draper's method (⁹) known as "IMMLEP protocol 1/79" and Mori's method (¹⁰), on the basis of the above criteria. Moreover, the activity of these M. leprae cells to stimulate host macrophage (M∅) cell functions which are important for the expression of host defense mechanisms against microbial organisms, especially intracellular parasites was also investigated.
Right : M. leprae purified by the method of Draper (IMMLEP Protocol 1/79) based on Percoll gra-dient centrifugation and aqueous two phase separation system and the method of Mori et al. based on the Percoll gradient ultracentrifugation were compared for some of their biological activities. The integrity of purified leprosy bacilli was higher in the latter preparation than the former. When the vaccines prepared from M. leprae cells obtained by the two purifica-tion methods by heat-treatment at 100°C were studied for their efficacy to modulate host macro-phage cell functions, the two vaccines were able to potentiate the phagocytic and active oxy-gen generating abilities but unable to augment the antilistierial activity of the host macropha-ges by double injections 21 days and 4 days before harvest via subcutaneous and intraperitoneal routes, respectively. M. leprae vaccine obtained by Draper's method more markedly height-ened the phagocytic activity of host macrophages than that prepared by Mori's meth d, where-as the inverse was the case for enhancement of the active oxygen producing ability of the host macrophages.

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/02/26 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Percoll密度勾配遠心法と水相2層分配法によるDraper法(IMMLEPプロトコール1/79)とPercoll密度勾配遠心法による森法とにより精製されたらい菌について若干の生物学的活性を比較検討した。精製らい菌のintegrityは後者において前者におけるよりも良かった。上述の2つの精製法によりえられたらい菌の100°C,熱処理ワクチンの宿主マクロファージ機能調節効果について検討した。その結果,2種のワクチンは細胞を採取する21日(皮下)および4日(腹腔内)前の2回注射により宿主マクロファージの貪食および活性酸素産生能を増強したが,抗リステリア活性を増強することは出来なかった。Draper法によりえられたらい菌のワクチンは森法により作られたものよりも宿主マクロファージの貪食活性をより高めたが,その活性酸素産生能の増強の点では逆の関係がみられた。

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