論文ID: OKD-128
For their exceptional beauty and great variety, waterlilies of the genus Nymphaea are popular ornamental plants worldwide. To improve their appealing traits, breeders worldwide have attempted intrasubgeneric and intersubgeneric cross-breeding and succeeded in producing new varieties. Molecular markers have become standard practice in the breeding process and for accurate variety identification. With increasing knowledge about transposable elements in eukaryotic genomes, retrotransposon-based markers have been developed for various plant species and were sometimes found to be more informative than conventional marker methods. Due to the lack of information about retrotransposons in waterlilies, this study aimed to confirm the presence and analyze the diversity of the Ty1-copia retrotransposons, which have been well studied and used to develop markers in many plants. 133 sequences of a conserved domain in the reverse transcriptase gene of Ty1-copia elements were isolated from 13 varieties of waterlilies and found to be homologous to either Ale, Angela, or TAR lineages. Sequences homologous to the Ale lineage were found to be abundant and diverse. Those homologous to the Angela lineage were found to be very conserved, but scarce. Those homologous to the TAR lineage were relatively conserved and were found in ample amounts. Furthermore, the results indicate that those homologous to the Angela or TAR lineages are more conserved to their counterpart in other plants than those homologous to the Ale lineage. From this study, 3 groups of Ty1-copia elements were found to be suitable candidates for development of retrotransposon-based markers for waterlilies in the future.
