Abstract
Root segments of plantlets (Aralia elata) grown by in vitro culture, were used as explants and cultured in the dark at 25 °C on MS medium supplemented with growth regulators. About 80-100 % of root segments formed calli on MS medium supplemented with 0.05-4.0 mg/l 2, 4- dichlorophenoxyacetic acid (2, 4-D). After 30 days of culture, calli formed on MS medium containing 0.05 mg/l 2, 4-D, were transferred on hormone-free MS medium and cultured at 25 °C in 16 h light (3,000 lux). Somatic embryogenesis was observed from the calli formed on medium supplemented with more than 0.5 mg/l 2, 4-D. Many somatic embryos and adventitious shoots were induced from the calli and developed into small plantlets by a subculture of 20-30 days interval. After acclimatization on vermiculite, regenerated plantlets were transplanted to a mixture of sandy masa soil/bark compost (3: 1, v/v). Nearly all plants were established and grew in to nursery stocks after 45 days.
