Lensless CMOS Imaging Device for Fluorescent and Non-Fluorescent Imaging Dedicated to Digital ELISA

Abstract

We report a lensless complementary metal-oxide-semiconductor (CMOS) imaging device that can obtain fluorescence and non-fluorescence images for digital enzyme linked immunosorbent assay (ELISA). In lensless imaging, it is important to manage the degradation of the spatial resolution with beam propagation. For fluorescence imaging, we applied an image processing technique based on deconvolution method to recover the spatial resolution. For non-fluorescence imaging, a collimated illumination is used and the detection error is reduced by using an angle scanning technique.