IEEJ Transactions on Sensors and Micromachines
Online ISSN : 1347-5525
Print ISSN : 1341-8939
ISSN-L : 1341-8939
Special Issue Paper
Sensitivity Improvement of Dielectrophoretic Impedance Measurement by Bacteria Concentration using Negative Dielectrophoresis
Yuki InoueMichihiko NakanoJunya Suehiro
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2016 Volume 136 Issue 5 Pages 148-152

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Abstract
Dielectrophoretic impedance measurement (DEPIM) is a method to detect bacteria in an aqueous solution. DEPIM consists of two simultaneous processes: dielectrophoretic trapping of the target bacteria onto a microelectrode and measurement of impedance change caused by trapping bacteria. In this study, a new bacterial concentration process was combined with DEPIM to improve its sensitivity. The concentration process was based on negative dielectrophoresis of bacteria caused by specific configuration of parallel electrodes. A ditched dielectric thin layer deposited on one of the parallel electrodes causes non-uniform electric field resulting in occurring negative dielectrophoresis. Two electrode configurations, one is for the bacteria concentration and the other is for DEPIM, were fabricated in a microchannel. As a result, the detection sensitivity of DEPIM increased about 1.6 times by combining the concentration.
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© 2016 by the Institute of Electrical Engineers of Japan
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