Simultaneous Analyses of Biochemical Components in Blood Using Infrared Spectroscopy Combined with Partial Least Squares

Abstract

Simultaneous quantitative analyses of biochemical substances were carried out using attenuated total reflection and Fourier transform infrared spectroscopy (ATR/FT-IR) combined with partial least squares. The concentrations of glucose, cholesterol, and triglycerides in human serum were measured simultaneously using the low-temperature ATR/FT-IR. The infrared absorption band of proteins in human serum measured at -2.4°C increased 5 times as large as that measured at room temperature. The increase was based on segregation of biochemical components during solidification process. The correlation coefficients between the concentrations determined by the low-temperature ATR/FT-IR and those determined by the conventional method were over 0.86. The fundamental investigation of simultaneous analysis of two enzymes, amylase and creatine kinase, was performed using ATR/FT-IR at room temperature. The changes in the concentrations of the products specific to the enzymes were measured simultaneously. The correlation coefficients between the concentrations of the products and the enzyme activities were over 0.95. The ATR/FT-IR combined with chemometrics would be a useful technique for a biochemical sensing system with a short turn-around time and easy maintenance.