IEEJ Transactions on Sensors and Micromachines
Online ISSN : 1347-5525
Print ISSN : 1341-8939
ISSN-L : 1341-8939
Development of a Microreactor for PCR
Seiji KondoNobuhiko MorimotoKunio HoriEtsuo ShinoharaKyoichi Kano
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Keywords: PCR, DNA, RNA
JOURNAL FREE ACCESS

1999 Volume 119 Issue 10 Pages 448-453

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Abstract

We have developed a microreactor for Polymerase Chain Reaction (PCR) by means of microfabrication technologies. The microreactor has been constructed to amplify DNAs, which was much faster than a conventional apparatus. Its size was 30×16mm, in which a reaction chamber with 10mm square and 300 μm depth was constracted. It was found that this microreactor requires only 20 minutes to perform 40 cycles of a shuttle PCR, 3 times faster than conventional apparatus.

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© The Institute of Electrical Engineers of Japan
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