1999 Volume 119 Issue 11 Pages 538-543
A carbon fiber fabric was modified by oxygen plasma treatment and glucose oxidase(GOD) was covalently immobilized on the modified carbon fiber by use of a condensing reagent. Specific activity of GOD immobilized on carbon fiber fabric was 51 U/mg at 30°C, pH 7. By using the GOD-immobilized carbon fiber fabric as a sensor, amperometric response to glucose was measured at a constant potential of +0.35V vs S. C. E under various pH and temperature conditions. Response current to glucose concentration was found to increase linearly with a slope of 1.8μA per 1mM glucose up to 70mM. The pH and temperature profiles of amperometric response of the GOD-immobilized carbon fiber fabric were similar to those of the activity of GOD.
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