A Method for the Determination of Plasma Renin Activity in Small Samples

Masashi IMAI; Hirofumi SOKABE

doi:10.1536/ihj.8.607

Masashi IMAI, Hirofumi SOKABE

著者情報

ジャーナルフリー

1967 年 8 巻 6 号 p. 607-619

DOI https://doi.org/10.1536/ihj.8.607

詳細

Published: 1967 Received: 1967/04/12 Available on J-STAGE: 2008/12/09 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2008/12/09 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : (1) Effects of Dowex 50W-X2 resin, diisopropyl fluorophosphate, and incubation at pH5.5 were compared for inhibiting angiotensinase activity in plasma with 0.93×10^-2M EDTA. We found that Dowex 50W-X2 is reliable.
(2) The method of Boucher et al. for determination of plasma renin activity using the Dowex resin was re-examined. We modified the procedures for the determination in small amount of samples.
(3) We measured angiotensinogen content of the plasma simultaneously, and expressed renin activity by the reaction constant of first order kinetics (K), since a linear correlation was found between amount of renin and the reaction constant.
(4) Evidences were presented that the cource of plasma renin-angiotensinogen reaction can be also approximated by the first order kinetics in our system.
(5) Recovery of angiotensin throughout the procedures was 74±12 (standard deviation)%. The results were sufficiently reproducible.
Right : In order to establish a reliable method for the determination of plasma renin activity in small samples, we have compared the methods reported. The method of Boucher et al. was found to be reliable, and modified in the laboratory. Expression of renin activity by the first order reaction constant was emphasized.

訂正日: 2008/12/09 訂正理由: - 訂正箇所: 著者情報訂正内容: Wrong : Masashi IMAI¹⁾²⁾, Hirofumi SOKABE¹⁾²⁾
Right : Masashi IMAI¹⁾, Hirofumi SOKABE²⁾

訂正日: 2008/12/09 訂正理由: - 訂正箇所: 所属機関情報訂正内容: 訂正前 :
1) Department of Pediatrics, Faculty of Medicine, University of Tokyo
2) Department of Pharmacology, Toho University School of Medicine

訂正後 :
1) the Department of Pediatrics, Faculty of Medicine, University of Tokyo
2) the Department of Pharmacology, Toho University School of Medicine

訂正日: 2008/12/09 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1. Davis, J.O.: Physiologist 5:65, 1962.
2. Peart, W.S.: Pharmacol. Rev. 17:143, 1965.
3. Boucher, R., Veyrat, R., De Champlain, J., and Genest, J.: Canad. Med. Ass. J. 90: 194, 1964.
4. Helmer, O.M. and Judson, W.E.: Circulation 27:1050, 1963.
5. Plentl, A.T. and Page, I.H.: J. Exp. Med. 78:367, 1943.
6. Bumpus, F.M.: Report at the International Symposium on Renal Hypertension, Cleveland, 1966.
7. Kawabe, K. and Sokabe, H.: Jap. Heart J. 6:51, 1965.
8. Harigaya, S., Sokabe, H., and Sakai, F.: Jap. Heart J. 7:66, 1966.
9. Yoshinaga, K., Aida, M., Maebashi, M., Sato, T., Abe, K., and Miwa, I.: Tohoku J. Exp. Med. 80:32, 1963.
10. Brown, J.J., Davies, D.L., Lever, A.F., Robertson, J.I.S., and Tree, M.: Biochem. J. 93:594, 1964.
11. Pickens, P.T., Bumpus, F.M., Lloyd, A.M., Smeby, R.R., and Page, I.H.: Circulat. Res. 17:438, 1965.
12. Warzynski, R., Demirjian, Y., and Hoobler, S.: Canad. Med. Ass. J. 90:225, 1964.
13. De Vito, E. and Fasciolo, J.C.: Acta Physiol. Latinoam. 15:129, 1965.
14. Gould, A.B., Skeggs, L.T., and Kahn, J.R.: Lab. Invest. 15:1802, 1966.
15. Yamaura, Y.: Shin Ikagaku (4th ed.), Nanzando, Tokyo, p. 296, 1964 (in Japanese).
16. Khairallah, P.A., Bumpus, F.M., Page, I.H., and Smeby, R.R.: Science 140:672, 1963.
17. Helmer, O.M.: Canad. Med. Ass. J. 90:221, 1964.
18. Veyrat, R., De Champlain, J., Boucher, R., and Genest, J.: Canad. Med. Ass. J.: 90:215, 1964.
19. Imai, M., Igarashi, Y., and Sokabe, H.: (in preparation).
20. Haas, E. and Goldblatt, H.: Circulat. Res. 20:45, 1967.

Right : 1. Davis, J.O.: Physiologist 5: 65, 1962.
2. Peart, W. S.: Pharmacol. Rev. 17: 143, 1965.
3. Boucher, R., Veyrat, R., De Champlain, J., and Genest, J.: Canad. Med. Ass. J. 90: 194, 1964.
4. Helmer, O. M. and Judson, W. E.: Circulation 27: 1050, 1963.
5. Plentl, A. T. and Page, I. H.: J. Exp. Med. 78: 367, 1943.
6. Bumpus, F. M.: Report at the International Symposium on Renal Hypertension, Cleveland, 1966.
7. Kawabe, K. and Sokabe, H.: Jap. Heart J. 6: 51, 1965.
8. Harigaya, S., Sokabe, H., and Sakai, F.: Jap. Heart J. 7: 66, 1966.
9. Yoshinaga, K., Aida, M., Maebashi, M., Sato, T., Abe, K., and Miwa, I.: Tohoku J. Exp. Med. 80: 32, 1963.
10. Brown, J. J., Davies, D. L., Lever, A. F., Robertson, J. I. S., and Tree, M.: Biochem. J. 93 594, 1964.
11. Pickens, P. T., Bumpus, F. M., Lloyd, A. M., Smeby, R. R., and Page, I. H.: Circulat. Res. 17: 438, 1965.
12. Warzynski, R., Demirjian, Y., and Hoobler, S.: Canad. Med. Ass. J. 90: 225, 1964.
13. De Vito, E. and Fasciolo, J. C.: Acta Physiol. Latinoam. 15: 129, 1965.
14. Gould, A. B., Skeggs, L. T., and Kahn, J. R.: Lab. Invest. 15: 1802, 1966.
15. Yamaura, Y.: Shin Ikagaku (4th ed.), Nanzando, Tokyo, p. 296, 1964 (in Japanese).
16. Khairallah, P. A., Bumpus, F. M., Page, I. H., and Smeby, R. R.: Science 140: 672, 1963.
17. Helmer, O. M.: Canad. Med. Ass. J. 90: 221, 1964.
18. Veyrat, R., De Champlain, J., Boucher, R., and Genest, J.: Canad. Med. Ass. J.: 90: 215, 1964.
19. Imai, M., Igarashi, Y., and Sokabe, H.: (in preparation).
20. Haas, E. and Goldblatt, H.: Circulat. Res. 20: 45, 1967.

訂正日: 2008/12/09 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

In order to establish a reliable method for the determination of plasma renin activity in small samples, we have compared the methods reported. The method of Boucher et al. was found to be reliable, and modified in the laboratory. Expression of renin activity by the first order reaction constant was emphasized.

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