抄録
Porphyromonas gingivalis has been implicated as a major pathogen in periodontal diseases. We previously succeeded in gene cloning of a 200-kDa membrane protein from P. gingivalis 381. The recombinant protein was immunoreacted by antisera from severe periodontitis patients. In this study, we attempted to identify the 200-kDa antigenic protein (200k-AP) using protein database. The recombinant 200k-AP was highly purified and digested with lysyl endopeptidase, and separated using reverse-phase high-pressure liquid column chromatography. Amino acid sequence of a chromatographic separated short peptide was examined and identified as VTVPENGK. By homology-search in protein database, the 8 amino acid sequence was exactly the same as the HagA which was known as useful immunogen to prevent colonization of P. gingivalis. This findings suggest that 200k-AP may be HagA and useful to develop the immunotherapy againstperiodontitis.
