IL-6 and IL-17 in the Gingival Crevicular Fluid During Orthodontic Root Resorption

抄録

Objectives : Root resorption is an unwanted effect of orthodontic tooth movement. An analysis of the cytokines in gingival crevicular fluid (GCF) is a potentially safer method of quantifying root resorption compared with conventional radiographic methods. This study aimed to quantify the levels of interleukin-6 (IL-6) and 17 (IL-17), released into the GCF during physiological root resorption and orthodontic tooth movement.
Materials and Methods : Subjects with severe root resorption (>1/3 of the original root length) were identified. A control group comprising subjects with no loss of the root structure or undergoing orthodontic treatment was also identified. Gingival crevicular fluid (GCF) was collected non-invasively from the mesial and distal sides of each of the four upper incisors by using filter paper strips. The eluted GCF was used for an enzyme-linked immunosorbent assay (ELISA) and a Western blot analysis. The antibodies used were specific for IL-6 and IL-17.
Results : The ELISA results with IL-6 and IL-17 antibodies showed statistically significant differences between the control group and the root resorption group. The Western blot analysis showed differential expression of IL-6 and IL-17 in the control and root resorption subjects.
Conclusions : These results confirm the presence of cytokines in the GCF of root resorbed subjects. The results highlight the potential for measuring IL-6 and IL-17 in the GCF as a biomarker to monitor root resorption.