抄録
A custom-made microarray for Porphyromonas gingivalis was constructed to investigate the gene expression profiling during its in vitro growth. There was a strong positive correlation between the results of both microarray and quantitative real-timePCR analyses (r=0.910, p<0.01). Many transcripts changed dramatically during the late-log to theearly stationary phase. In a stationary phase, most of the transcripts decreased. However, transcripts related to the transposon function, oxidative stress, and energy metabolism were increased or sustained at high levels. More notably, the transcripts related to protein folding and protein stabilization, such as dnak, groEL, groES, and htpG, increased significantly after 10h in the stationary phase, suggesting that these chaperone proteins may play an important role in bacterial survival. All together, our custom array is useful for transcriptional research for P. gingivalis. It provides that the gene expressions are growth phase-specific and are changed dramatically in response to environmental change during bacterial culture.
