Abstract
Metallic cobalt (Co), zinc (Zn) and lead (Pb) were more easily dissolved in albumin and glycine water solution than in water. The solubility of these metals in albumin and glycine solution increased with increase of the concentration. The tested Co and Zn samples apart from cobalt oxide (CoO) were more soluble in fetal calf serum than in water. In the case of Pb, the tested Pb samples apart from lead dioxide (PbO2) were less soluble in serum and Tyrode's solution than in water. In the case of Co and Zn samples, there were no significant differences in solubility between water and Tyrode's solution.
When Co or Zn dissolved in protein-, amino acid-or disodium ethylenediaminetetraacetate (NaEDTA)-Tyrode's solution was added to the cultures, the dissolved Co or Zn did not exert a toxic action on FM3A cells in proportion to the amount of Co or Zn. On the other hand, Pb dissolved in protein or amino acid water solution showed a toxic action on FM3A cells in proportion to the amount of Pb, but Pb dissolved in NaEDTA water solution did not exhibit a toxic action on FM3A cells.
