Abstract
A new spectrofluorometrical method is proposed for the determination of trace of 3, 4-benzpyrene in the benzene solution contaminated with 3, 4-benzfluoranthene or 1, 2-benzpyrene and 3, 4-benzfluoranthene. This method consists of the removing of oxygen dissolved in a test solution, measurement of fluorescence spectrum and the determination by narrow base line method proposed by Commins for the spectrophotometrical determina-tion of polynuclear hydrocarbons. By this method 3, 4-benzpyrene can be determined accurately even in the solution containing 3, 4-benzpyrene, 3, 4-benzfluoranthene and 1, 2-benzpyrene in a concentration ratio of 1 : 100 : 100.
