Internal Medicine
Online ISSN : 1349-7235
Print ISSN : 0918-2918
Hydrogen May Inhibit Collagen-Induced Platelet Aggregation: An ex vivo and in vivo Study
Satoru TakeuchiKojiro WadaKimihiro NagataniHideo OsadaNaoki OtaniHiroshi Nawashiro
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2012 Volume 51 Issue 11 Pages 1309-1313


Objective Hydrogen selectively reduces hydroxyl radicals and peroxynitrite, and numerous experimental and clinical studies suggest that hydrogen can exert potent cellular protective effects against a wide variety of diseases. Furthermore, there is increasing evidence that antioxidants can modulate platelet activation. The aim of the present study was to investigate the relationship between hydrogen and collagen-induced platelet aggregation.
Methods For human ex vivo studies, we collected blood samples from six healthy humans and added normal saline or hydrogen-rich saline to blood and platelet-rich plasma. We found that collagen (1 μg/mL)-induced platelet aggregation was significantly inhibited by hydrogen-rich saline compared with a normal saline group (p=0.044). For rat in vivo studies, animals (n=17) were exposed to either nitrogen-based mixed gas with hydrogen (H2 gas group; n=9) or without hydrogen (non-H2 gas group; n=8). Additionally, another animals (n=13) administered either normal (NS group; n=7) or hydrogen-rich saline (HS group; n=6) (5 ml/kg) via intravenous infusion. Blood samples were drawn from the vena cava before treatment and from the right ventricle after treatment. Collagen (12 μg/mL)-induced platelet aggregation was then measured.
Results Collagen-induced platelet aggregation was significantly decreased in H2 gas and HS group rats (p=0.042, 0.018, respectively), while there was no difference in non-H2 gas and NS group rats before and after treatment.
Conclusion In summary, these data suggest that hydrogen may inhibit collagen-induced platelet aggregation.

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© 2012 by The Japanese Society of Internal Medicine
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