Abstract
Due to kidney tuberculosis one kidney of the patient has been surgically exstirpated. He has been treated with PAS which resulted an apparent arrest of the disease for some time then the patient was admitted to the 2nd Tokyo National Hospital.
He was given 1g of SM twice a week and 0.5g INH daily for 2 years, however, the urine was constantly positive for tubercle bacilli. 3% of the urine test the stain (Nakasima) only grew in Ogawa's medium containing 1, 000mcg per ml of streptomycin.
The growth appeared within 3-5 weeks in the presence of 1, 000mcg of SM per ml of medium, but not in the tube without SM and in concentration of 1, 10 and 1, 000mcg of SM per ml of medium.
If it concubated more than 7 weeks, a slight growth was observed at the lower concentration of SM of medium.
The experiments were performed in vitro and in vivo.
Nakasima strain required the presence of SM for about 200mcg per ml to 1, 500mcg per ml in the medium. This strain was susceptible to INH, PAS and Kanamycin. A large doses of this strain were subcutaneously inoculated into gineapigs, but the disease did not occur on the animals as long as they were not given SM.
And these animals showed a slight virulence when intramuscularily they were given 100mg of SM daily.
Owing to the fact that more than one year after the SM therapy the patient's urine was bac. positive, it seemed that this strain would be dependent not on SM itself but on SM like substance.
