Abstract
Inactivating effects of two bactericidal surfactants, Sarabon-Di (octylamino ethyl) glycine hydrochloride (10w/v%) in polyoxyethylene alkylphenyl ether (3w/v%), and Osvan-Dimethylbenzyllauryl ammonium chloride (10w/v%), against polio virus type I (Mahoney strain) and adeno virus type 3 have been tested.
The results obtained were as follows:
1) 1ml of 1:125 diluted sarabon with Hanks' BSS was added to 1ml of polio virus cultivated medium (LY-BSS with 3% inactivated chick serum and streptomycin and kanamycin 100mcg/ml each) containing 106TCD50/ml of virus. The mixture was pipetted well and devided into two parts and each one was kept at 20°C and 40°C for one hour. Ten-fold dilution using BSS was made in these mixtures and six diluted materials were inoculated to 6 sets of three HeLa tubes in which the doses of polio virus were estimated to be 5×103TCD50/ml, 5×102TCD50/ml, and 5×10TCD50/ml.
All inoculated HeLa tubes showed 3-4 cytopathic effects which appeared to be due to polio virus within a week after inoculation.
2) The same amount of virus cultivated medium containing 104TCD50/ml of adeno virus type 3 and a diluted sarabon (1:2, 500) were pipetted well and the mixtures were kept at 20°C and 40°C for one hour, Ten fold dilution of virus-sarabon mixtures were made and four sets of diluted materials were inoculated to HeLa tubes in which doses of virus were estimated to be 5×102TCD50/ml and 5×10TCD50/ml.
In these series of experiment HeLa cells also revealed typical cytopathic effects due to adeno virus.
3) Using similar method to the above experiments the mixtures of polio virus suspension and diluted osvan (1:1, 000), adeno virus suspension and diluted osvan (1:500) were made. They were kept at 20°C for one hour, and the mixtures were diluted and inoculated to the HeLa tubes. In these instances, all HeLa cells developed typical CPE due to viruses used.
4) From these data the conclusions will be drawn that both sarabon and osvan do not have much potency of inactivating effects against polio and adeno viruses in spite of effectiveness of these surfactants to various pathogenic organisms such as staphylococci, dysentery bacilli, salmonella and candida albicans and so on.
