Abstract
After the various experimental conditions on the fractional analysis of serum protein by salting out method using phosphates were investigated; the values determined by this method were compared with those by Tiselius's electrophoretic method.
The results of these investigations were as follows:
1) As the turbidities corresponding to total protein or globulin, i. e, extinctions E1 or E2, and the A/G ratios calculated from these data, increase with time interval, the measurement should be performed within 30 minutes after developing the turbidities.
2) While the extinction E1 and E2 at 37°C increase as much 17% as at 15°C, the average A/G ratio calculated at the same temperature decrease to 90% of the values at 15°C.
3) The relative error at each measurements is ±11-3%.
4) The extinction E1 and the concentration of to total protein correlates closely, i. e. the correlation coefficient is 0.89. Therefore, as long as the measurements of the protein content of unknown Samples are performed in parallel with the known, the content of the former are able to determine approximately by turbidimetry.
5) The correlation coefficients of the procentual concentration of each protein fraction between measured by this and by Tiselius's method are: albumin, 0.89; α, β and γ globulin, 0.58, 0.90 and 0.87 respectively, These figures are considered to be in good agreement except γ-globulin.
Because of the fractional analysis of plasma protein by salting out method using phosphates is simple and rapid to perform, and moreover the values obtained by this method correlates closely with those by Tiselius's method it seems to be suitable as a routine method for the fractional analysis of serum protein.
