抄録
In an established solid MH134 tumor in C3H/He mice, necrotic part increased in size after a time while viable part got smaller, At this stage, many lymph node metastases emerged stimulating the compensatory growth for the reduction of viable part. This fact appears to suggest that a well-established tumor bearing host can sustain a certain amount of viable part of tumor and that the emregence of metastases is, to some extent, delayed if the reduction of viable part is prevented. In fact, it was recently found that the viable part contains substances, inhibiting DNA-synthesis in MH134 cancer cell.
In an attempt to inhibit metastasis and recurrence, the primary tumor was removed and the viable part of this tumor was incubated for 7 hours under 37°C in 7% bicarbonate solution and was autotransplanted. The effect of 7% bicarbonate solution on the cancer cell is as follows:
1) According to our experiments in vitro, the bicarbonate treated cancer cell reduces its O2 consumption reversibly. This fact indicates that the enzymatic activity of the bicarbonate treated autograft continues in the host for a while.
2) It prevents the reduction of tumor-graft that otherwise should have occurred in a well established MH134 tumor. This fact indicates that it stabilizes the viable part, releasing the inhibitor continuously against dispersed cancer cells in the tumor bearing host.
3) It has no effect upon the inhibitor of the viable part in vitro.
4) It inhibits the growth of tumor cell.
The result of this treatment was as follows:
(I) 149 mice bearing 2 to 3 weeks old MH134 tumor underwent removal of the tumor. 77 of them received bicarbonate-treated tumor autograft and 72 none. Survival rate during 300 days period after operation was threefold higher in the treated than in the controls. For example after 90 days it was 14/77(18.6%) in the treated and 4/72(5.6%) in the controls.
(1) In C3H mice of spontaneous mammary cancer, survival rate of the thus treated was 5/14 (35.7%) while 0/11 (0%) in the controls 154 days after radical mastectomy.
Autotransplantation of bicarbonate-treated tumor did not reverse the fall of serum albumin and the increase of alpha globulin, but it did rectify, to some extent, the fall of liver catalase activity the increase of serum lactic dehydrogenase and the involution of thymus. In such an occasion, splenomegaly was noted with accompanying lymphopoesis. Some clinical experience on 20 gastric cancer patients who have received this treatment is to be presented.
