抄録
In the study of the cultivation of tubercle bacilli in patient's feces I have found following facts and succeeded in developing a new cultivation method by the use of 0.8% Marzonin (Sodium Ethylmerculithiosalicylate) and 4% Cautic Soda.
1) On cultivation of tubercle baccilli in feces, the most common contaminating organisms are bacilli and cocci of various kinds. Fungi are next in common.
2) The number of various contaminating organisms which grow in the course of cultivation of tubercle bacilli varies according to an individual factor. However, in feces of those who are subject to have soft or diarrheal stools it is observed, when cultured, a large number of colonies of various kinds of bacteria on agar plate, and a tendency of marked growth of contaminating organisms.
3) The growth of the contaminating organisms may be checked by giving the patient a daily dose of sulfanilamide for several days before the cultivation.
4) This new tubercle bacilli cultivation method which I have developed and which I call “Marzonin-alkali method” is superior to any other known methods both in securing the number of tubercle bacilli colony and in inhibiting a contamination.
