抄録
Genetic diversity among 88 accessions of Pinanga javana were revealed in six natural populations in Mt. Sawal, Ciamis (JW327); Mt. Ciremai, Kuningan (JW329); Mt. Slamet, Purwokerto (JW331); Mt. Salak, Bogor (JW334); Mt. Pangrango, Bogor (JW337); and Mt. Halimun, Bogor (JW339), all in very restricted area only in lower montane forest from 800-1700 m elevation in Java, Indonesia by Random Amplified Polymorphic DNA (RAPD) markers. Natural populations of the species have been decreased dramatically because of habitat conversion and over-exploitation. Ten of 40 primers were reproducible among the 88 accessions of P. javana and plotted total 100 bands, of which 51 were polymorphic. The genetic similarity according to Jaccard's coefficients among accessions ranged from 0.747 to 1.000 and the mean genetic similarity among populations ranged from 0.811 to 0.922. The UPGMA analysis showed two clusters: “A” of JW334, JW339, JW337, JW329 and JW327; and “B” of JW331. The cluster “A” was subdivided into two subclusters; one of JW334, JW339, JW337 and JW329 and the other one of JW327. These results provided intra- and inter-populational genetic diversity of P. javana. The present observation suggested that both in situ and ex situ conservations were necessary for the genetic diversity of P. javana, especially the populations in Mt. Slamet and Mt. Salak because of their narrow genetic diversity. Moreover, the species was susceptible for habitat conversion, would never grow in any disturbed forest and has become rare in the nature.
