Fluorescence in situ hybridization and genomic in situ hybridization to identify the parental genomes in the intergeneric hybrid between Chrysanthemum japonicum and Nipponanthemum nipponicum

抄録

Chrysanthemum japonicum (2n=18; ?? ) and Nipponanthemum nipponicum (2n=18; ?? ) were artificially crossed and produced healthy F₁ hybrid plants by aids of the series of embryo rescue, axenic culture and acclimatization. FISH biotinlabeled probes of 5S rDNA and pTa71 for 45S rDNA distinguished 5S rDNA and NOR regions of the chromosomes in the genome of respective species in the chromosome complement of the F₁ hybrid. One 5S rDNA-signal and two 45S rDNA-signals were shown in the nine chromosomes of C. japonicum genome in the F₁ hybrid chromosome complement (2n=18), while no 5S rDNA-signal and one 45S rDNA-signal was shown in the nine chromosomes of N. nipponicum genome. GISH differentiated the genome of C. japonicum and that of N. nipponicum in the F₁ hybrid chromosome complement using the probe of total genomic DNA of N. nipponicum and showed that the parental genomes in the chromosome complement of the hybrid were generally in separate domains during mitosis.