Chromosome Botany
Online ISSN : 1881-8285
Print ISSN : 1881-5936
ISSN-L : 1881-5936
Regular Article
Chromosomal aberrations and Ac/Ds transposition in Garlic
Rasha Kamal Helmey Gehan Mohamed Anwar
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2018 年 12 巻 4 号 p. 72-76

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Garlic (Allium sativum L.) is a commonly used Allium crop worldwide. It might be expected that garlic would show some intraspecific variations because of its vegetative reproduction. This study shed light on such variations joined with the existence of the transposable element Ac in garlic. The examined cells exhibited different types of chromosomal aberration were recorded as percentages of the total abnormal cells. Microscopic examination of root tip cells of the (Egaseed 2) clone showed a variety of chromosomal aberrations represented as: gap, chromatid deletion, chromatid break, end-to-end association and centromeric attenuation. DNA was isolated from ten individual cloves of Egaseed 2 clone. Using Ac primer, a monomorphic fragment of 100 bp was detected in 10 cloves of the Egyptian clone (Egaseed 2) of garlic. One of the same cloves, no. 7, has an additional fragment of 300 bp. The relationship between Ac element existence and chromosomal changes occurrence was discussed.
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© 2018 by the International Society of Chromosome Botany
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