マウスLAK細胞の細胞内画分の細胞傷害性に関する研究

抄録

The cytotoxic activity of subcellular fractions in LAK cells derived from normal spleen cells of inbred WHT/Ht mice was investigated. The cytotoxic activity of LAK cells,determined by ⁵¹Cr-release assay with syngeneic tunlor cells as a target, was found to reach the maximum level of 44.2% at the 5 th day of cultivaton with 8,000 units of rlL-2/ml culture. LAK cells lysed by sonication under hypotonic conditions were fractionated into 4 subcellular fractions. The mitochondrial fraction contained a component which, even at low concentration, had an immediate cytotoxic effect. The microsomal and cytosol fractions also contained a component which acted with a larger quantity of proteins and a longer period of incubation time than the factor of the mitochondrial fraction. These observations suggested that at least two types of cytotoxic sub-stances, which have different subcellular localizations and mechanisms of action, are present in LAK cells.