ウサギ VX2 移植頬粘膜癌の増殖に伴うリンパ管の構築と所属リンパ節への転移経路に関する組織学的研究

抄録

Carcinoma of the buccal mucosa is likely to metastasize to cervical lymph nodes if it has developed to surrounding tissues, and prognosis is poor. In the VX2 carcinoma transplanted to the buccal mucosa in the rabbit, lymphatic architecture due to proliferation of the tumor, the routes to regional lymph nodes and metastatic lymph nodes were examined. VX2 carcinoma, subcultured in the rabbit femoral region, was transplanted to the right buccal mucosa near the occlusal line. The architecture of collecting lymphatic vessels in the surrounding tissues of the tumor and metastasis to the regional lymph nodes were histologically examined by intravascular injection of India ink and impregnation with silver nitrate solution, 3, 7 and 14 days after the tumor transplantation, and routes to the regional lymph nodes were traced by the stick injection of India ink.At 3 days after tumor transplantation, diameters of collecting lymphatic vessels were markedly dilated in the nearby carcinomatous area, and lymphatic vessels showed a tendency of tortuousness 7 days after tumor transplantation. At 14 days after tumor transplantation, formation of new lymphatic vessels was noted in the pericarcinomatous area, and these lymphatic vessels running adjacent to each other formed a network. The routes to the regional lymph nodes in the tumor transplanted side were clearly detected, and no lymphatic routes to the opposite side were seen. Fourteen days after tumor transplantation, the metastasis in cervical lymph nodes was observed in peripheral sinuses near afferent lymphatic vessels.Thus, it was suggested in this study that there were changes of lymphatic architectures, due to proliferation of the VX2 carcinoma transplanted into the buccal mucosa, and metastatic routes to the regional lymph nodes in rabbits.