Abstract
Periodontal disease is a major public health problem that affects over half of the adult population worldwide. Lipopolysaccharides (LPS), produced by the periodontopathic bacterium Porphyromonas gingivalis, induces the expression of inflammatory cytokines that promote inflammatory bone destruction. Buckwheat bran (BB), a traditional pseudocereal crop, belongs to a group of grains having high nutritional value and has been reported to be an effective agent. However, the anti-inflammatory effects of BB on Porphyromonas gingivalis-lipopolysaccharide (P.g.-LPS) -stimulated human gingival fibroblasts (HGFs) have not been completely elucidated yet. This study determined that the buckwheat bran flour hydrolysate powder (BBFHP) significantly inhibited the P.g.-LPS-induced production of inflammation-associated genes, including interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α) and Toll-like receptor 4 (TLR4). Moreover, BBFHP suppressed P.g.-LPS-induced NF-κB signaling. The results suggest that the BBFHP attenuates the production of inflammation-associated molecules and suppresses the activation of NF-κB in P.g.-LPS treated HGFs.
