Rapid Methods for Determination of ATP related Compounds in muscle by Low Pressure Column Chromatography
1987 Volume 13 Issue 4 Pages 139-145
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1987 Volume 13 Issue 4 Pages 139-145
The rapid methods for determination of ATP related compounds in muscle by low pressure column chromatography have been studied.
The rapid separation was achieved at a high flow rate of 3 ml/min, by using a Perista Minipump and a small column (φ5 mm×20 mm) packed with an anion exchange resin (AG 1×4, -400 mesh, Cl type).
This made it possible to fractionate ATP related compounds into HxR+ Hx, AMP, IMP, ADP and ATP in about 25 min by the concave gradient elution system. The hydrochloric acid-sodium chloride concentration gradients were prepared with two and three chambered gradient apparatuses.
When the flow rate was reduced to 2 ml/min, the time required for the separation increased to 40 min, but a better separation of each component was obtained at this flow rate.
