Antioxidative Activities of Pigments from Dried Marine Algae

Abstract

Pigment from dried 6 marine algae (one species of green, three of brown and two of red algae) with acetone were investigated with antioxidative activity by the Warburg method. And at the same time, it was measurement with pH, absorption, thin layer chromatography and reducing power. The following results of this investigation were obtained :
1) Antioxidative activities of pigments from dried marine algae were shown with Aonori, Wakame, Asakusanori, Makonbu, Hijiki and Tengusa.
2) The yellow and blue absorption maxima of pigments with acetone were in the range of 425-419nm (Chlorophyll a, b) and 672-675nm (Chlorophyll a, b).
3) Pigments from dried marine algae with acetone were showed with green by green algae, yellow-green by brown algae and green, gray-green by red algae.
4) Thin layer chromatography (the solvent system was CHCl3 : MeOH : AcOH (90 : 10 : 1) of Silicagel-B5 of pigments from dried marine algae was found by 5 fractions in Wakame, 4 fractions in Aonori, Makonbu and Hijiki, and 3 fractions in Asakusanori and Tenguse.
5) Strongest antioxidative activities of fractions by thin layer chromatography were showed with higher front fraction in all pigments.