Abstract
The influence of the high-pressure treatment on the activity of the trypsin and chymotrypsin was examined. Both enzymes showed the remaining activity of about 92% after the high-pressure treatment of 600MP and both enzymes showed a very high pressure resistance.
The Km value of both enzymes showed almost constant values. It was suggested that Vmax decrease after the high-pressure treatment.
Moreover, it was showed that the second structure of enzyme had been changed from the result of CD and NIR by the high-pressure treatment of 600MPa. The results of UV spectrum and hydrophobicity strongly suggested that the enzymes' surface structure were changed after pressure treatment. It was found that the reactive behavior of both enzymes had been changed because the structure around the catalytic site of the surface structure of the enzymes been influenced by the high-pressure treatment of 600MPa.
