Abstract
Enterococcus faecium MR006 was isolated from Miso and found to produce a bacteriocin with strong antibacterial activity. A culture of E. faecium MR006, maintained at a constant pH of 6.0 or 7.0 at 30°C, exhibited bacteriocin activity two-fold higher than that of the same culture grown under pH-uncontrolled conditions. MR006 bacteriocin was purified to homogeneity on SDS-PAGE by hydrophobic column chromatography and gel filtration. The estimated molecular weight of the MR006 bacteriocin was approximately 5.5 kDa, based on the tricine-SDS-PAGE system. Amino acid sequencing analysis revealed that the bacteriocin was identified to enterocin B, which is a bacteriocin produced by E. faecium T136.
