日本法科学技術学会誌
Online ISSN : 1881-4689
Print ISSN : 1880-1323
ISSN-L : 1880-1323
技術報告
TaqMan プローブ PCR 法による ABO 式血液型分類に関する一考察
尾形 早映子片桐 秀信小林 舞子宇井 勇人吉井 富夫
著者情報
ジャーナル フリー

2007 年 12 巻 2 号 p. 167-176

詳細
抄録
  We developed a highly sensitive and rapid ABO genotyping method based on the polymerase chain reaction (PCR) using TaqMan kit, in which three single nucleotide polymorphisms (SNPs: positions no. 261, 796 and 803) were targeted and searched with original probes. This method enabled us to confirm genotypes OO, AB (10 samples each), AO, BO, AA, and BB (1 sample each) and phenotypes A and B (10 samples each). All procedures from PCR took only 2 hours and 30 minutes. The TaqMan probe PCR method required only 1.2 ng of template DNA for ABO genotyping. It also allowed us to confirm the presence of PCR inhibitors and to estimate the quantity of template DNA by using real-time PCR, which would be utilized in subsequent examinations, as with the Multiplex STR method.
著者関連情報
© 2007 日本法科学技術学会
前の記事 次の記事
feedback
Top