Abstract
A quantitative determination by a simple detection system for carbohydrates with elution chromatography was developed. This method depends upon the detection of ultraviolet absorbing chromophere, with absorption maximum in the 260-270 mμ region, produced by heating the carbohydrates in the eluent solution without reagent. In the product from fructose, the existence of furfural and formic acid was confirmed . The heating condition and eluant buffer (conc., pH) affected the intensity of ultraviolet absorption produced. Under the condition of heating below 190°C and shorter than 20 min, the greater efficiency was obtained at higher temperature and longer period . Higher concentration and neutral pH of borate buffer were more effective . The molar absorption coefficient at 260 mp of mono- and oligosaccharides heated in a solution of 0.8 M borate buffer pH 7.0 for 9.5 min at 190°C was 500-3000 . The coefficient of variation of this method was less than 3.5%. A heating period was obtained by adjusting the diameter and length of teflon tube connected with the chromatographic column. In order to decrease the extra column effect, it was effective to decrease the diameter of tube (0.5 mm) and heating period, and increase the heating temperature . Several examples for mono- and oligosaccharides and oligodextrines were shown.
