Abstract
The action of cyclodextrin glucanotransferase (CGT-ase) on α-glucosylfluoride (α-GF) or α-maltosylfluoride(α-G2F)in the presence of 9-cyclodextrin(CD)or α-CD, respectively, was investigated for the purpose of γ-CD production. The optimum condition of the γ-CD production from the mixture of α-GF and β-CD by CGT-ase was α-GF: 30 mM, β-CD: 10 mM, CGT-ase from Bacillus megaterium: 60 unit/ml, incubation at 40°C for 30 min. In this condition, the amount of γ-CDproduction was 1.7 mM and the ratio of α-and γ-CD production was 1 : 2. In the reaction system of α-G2F and α-CD, CGT-ase from B, macerans produced more γ-CD than did the enzyme from B. megaterium. But the ratio of α-, β-, and γ-CD formation was almost the same as that from starch.
