Journal of the Japanese Society of Starch Science
Online ISSN : 1884-488X
Print ISSN : 0021-5406
ISSN-L : 0021-5406
Continuous Conversion of Cyclodextrin to Maltooligosaccharides with Immobilized α-Amylase
Shigeo SAKAIShusaku YOSHIDAHiroshi ISHIGAMIKozo HARA
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1991 Volume 38 Issue 1 Pages 17-22

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Abstract
A new carrier resin (FE-4611) was synthesized, on which α-amylase was immobilized. FE-4611 is a strongly hydrophilic resin of macroreticular type. α-Amylase was immobilized on FE-4611 and 3 species of Chitopearl. Continuous conversion of CDs into maltooligosaccharides with these kinds of immobilized a-amylase was investigated . The α-amylase immobilized on FE-4611 could keep up its effective performance over one month. This carrier resin bearing α-amylase was effectively rejuvenated for reuse by use of warm alkali solution . By contrast, α-amylase immobilized on Chitopearl lasted only for about two weeks . It was impossible to rejuvenate the enzyme immobilized on this carrier.
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© The Japanese Society of Applied Glycoscience
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