Abstract
α-Glucan composed of only D -glucose was extracted with boiling water from the fruit body of Grifola frondosa and purified through the columns of DEAE-cellulose (borate form) and Sepharose CL-4B. The polysaccharide was found to be a glycogen-type from the results of methylation study and enzymic hydrolysis. About 1.3×106 of weight-average molecular weight and 540 α-1, 4 linked unit chains were revealed by gel chromatography of pullulanase and isoamylase digest of the polysaccharide.
