Bioactive Compounds from the Leaves of Lumnitzera racemosa against Acetaminophen-induced Liver damage in vitro

Abstract

Phytochemical investigation of the mangrove plant Lumnitzera racemosa WILLD (Fam: Combretaceae) has resulted in the isolation of 8 compounds by various chromatographic techniques (silica gel, ODS column chromatography and HPLC). The structures of these compounds were determined by spectrometric analysis (UV, IR, HR-ESI-MS, 1D and 2D-NMR). All the isolated compounds were evaluated for their hepatoprotective activity. The Compound number 8 showed high hepatoprotective activity against acetoaminophen and compound 1 showed moderate activity compared to glycyrrhizin as the positive control using human Hep G2 cell line. The Compounds number 2, 3, 4, 5, 6 and 8 showed the highest DPPH radical scavenging activity (IC50: 14.74±5.66, 18.88±3.56, 5.93±2.1, 7.17±0.42, 7.38±1.03 and 15.72±1.18 µM, respectively), while compounds 1 and 7 showed lowest DPPH radical scavenging activity comparable with the standard trolox (IC50: 5.93±2.19 μM).