抄録
We reported that the primary stage of calcification on the bone/titanium interface was affected by the amount of radiation exposure. In this study, we used pure Ti and treated Ti and observed the primary calcification on the surface of the materials when osteoblasts were irradiated in vitro.
Pure titanium disks were employed. Some samples were anodized in an electrolytic solution and hydrothermal treatment was conducted at 300℃ for 2 hours. Bone marrow cells were obtained from Wistar rats. For primary cultures, the femora was removed and washed with α-Minimal Essential Medium. Aliquots of the rat bone marrow cell suspension were cultured for 5 days and irradiated. The first subcultures were maintained up to 14 days. Cultures were fixed, dehydrated through a graded series of ethanol, and freeze dried with t-butyl alcohol. Specimens were observed either under SEM or an electron probe microanalyser. Furthermore, some specimens were used for quantitative analyses.
Irradiation doses under 0.4 Gy induced no significant changes of the primary stage of calcification on the bone/titanium interface. Calcification rates for 4 Gy were significantly different from the control and under 0.4 mGy samples. Calcification rates for treated titanium were significantly different from pure titanium under 0.4 Gy. （198 words）
