2017 Volume 66 Issue 5 Pages 524-529
In this study, we evaluated the performance of XN-3000, an automated hematology analyzer, in measuring cell count in cerebrospinal fluid samples. The coefficients of variation (CV) and mean value in the test for within-run reproducibility for total WBC count were 2.1% (103 cells/μL) and 6.2% (25 cells/μL), respectively. The limit of detection was estimated to be 2 cells/μL. The regression equations for the counts of total WBCs, mononuclear cells, and polymorphonuclear cells between XN-3000 (y) and the visual observation method using the Fuchs–Rosenthal counting chamber (x) were y = 0.96x + 1.51 (r = 0.997), y = 0.98x (r = 0.995), and y = 0.95x + 1.18 (r = 0.999), respectively. The results showed almost good correlations. In the scattergram, the cluster of Cryptococcus spp. was displayed in a debris area, which enabled the precise cell count. Furthermore, we may detect Cryptococcus spp. by using an automated analyzer. Measurement with an automated analyzer is useful, especially in the evening and during holidays, and anybody can report an examination result quickly and correctly.
