2021 Volume 70 Issue 2 Pages 325-329
The rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) is important. In this study, a rapid detection method using MRSA-LA with culture fluid from positive blood culture bottles (direct method) was compared with an antimicrobial susceptibility test and real-time PCR assay. Thirty-seven blood culture isolates that were identified as S. aureus were used. Results showed that 11 isolates were determined to be MRSA and 26 were found to be methicillin-susceptible S. aureus (MSSA) by the antimicrobial susceptibility test. On the other hand, twelve isolates were determined to be MRSA by the direct method and PCR assay. In other words, one bottle with a mixture of MSSA and methicillin-resistant Staphylococcus epidermidis (MRSE) was determined to have MRSA. In conclusion, the detectability of MRSA from blood culture by this method was satisfactory.
