Cell-adhesive Protein Immobilization Using Tresyl Chloride-activation Technique for the Enhancement of Initial Cell Attachment

抄録

The aim of this study was to evaluate the biological effect of immobilized cell-adhesive protein onto titanium using tresyl chloride-activated technique. Titanium sputter-coated glass (Ti-glass) was used as a substrate. Tresyl chloride was directly applied onto Ti-glass. Fibronectin or collagen was immobilized to tresylated Ti-glass. The measurement of X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared reflection-adsorption spectroscopy confirmed the immobilization of fibronectin or collagen onto tresylated Ti-glass. The immobilization of fibronectin was not effective for enhancing the initial attachment of human gingival fibroblasts, but collagen immobilization was effective. The observation of scanning electron microscope also revealed that collagen immobilization affected the morphological changes of attached human fibroblast. In conclusion, the present study supported that collagen immobilization onto Ti-glass using tresyl chloride-activation technique improved the activity of human gingival fibroblasts during initial attachment.