Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
ORIGINAL ARTICLES
In vitro Osteogenic Differentiation Potential of Dental Pulp Stem Cells
Masaharu TAKEYASUTadashige NOZAKIMasatoshi WATANABEMitsuko SHINOHARAJoji MORITAAtsushi HIDAKAKazuya IWAMOTOToshifumi TAKAHASHISachiko NAGATAMichiharu DAITOKiyoshi OHURA
著者情報
キーワード: dental pulp, differentiation, bone
ジャーナル フリー

2004 年 2 巻 1 号 p. 25-30

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In the present study, the in vitro osteogenic differentiating potential of rat dental pulp stem cells was examined. The induction was carried out under the same inducing system as used for bone marrow cells. Alkaline phosphatase (ALP) activity and mineralization were measured at 1, 2, 3, and 6 weeks after the induction. The level of ALP activity was very high at 1 week after induction and the level was maintained for 3 weeks, however the level of ALP activity was dramatically decreased (to 50%) after 6 weeks. Although mineralization was detected at low levels after 3 weeks, it became expanded to up to 29 nodules mineralized at 6 weeks after induction, as determined by von Kossa staining. It was shown that in vitro osteogenic differentiation of rat dental pulp stem cells was induced following the increase of the ALP activity at an early stage of the induction.

著者関連情報
© 2004 by Japanese Association of Regenerative Dentistry
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