2024 年 22 巻 1 号 p. 23-28
Dental desensitizing materials are often applied to wedge defects, potentially allowing the material to come into contact with pulp cells. In this study, we aimed to clarify cell proliferation, calcium dynamics, and expression of integrin αvβ3 by culturing cells in a medium containing a calcium carbonate-based dental hypersensitivity treatment agent.
In this experiment, we performed a WST assay and observed fluorescently labeled antibodies using a confocal laser scanning microscope. The number of cells increased in the experimental group compared to the control group on day 1 of incubation. On days 4 and 7 of incubation, material-derived calcium was localized in the cytoplasm of the experimental group, overlapping with the Integrin αvβ3 expression sites. It was suggested that calcium derived from the material is taken up into the cytoplasm and promotes cell proliferation in the early stages of culture.
