Effect of Flowable Composite Resin on Cell Viability of Balb/c 3T3 Cells

抄録

The flowable composite resins have been routinely used in recent clinical dentistry. However, few studies have investigated the biological influence of flowable composite resins commercially available in Japan. In this study, we compared cell viability among 5 products currently available in dental clinical practice. Using mouse-derived Balb/c 3T3 cells, DMEM containing 5% fetal calf serum was adopted as an assay medium. Initially, the flowable resins were dissolved in the assay medium in the presence of dimethyl sulfoxide (DMSO) be-fore photopolymerization, and each solution was diluted multiple times to investigate cell viability.

As a result, all products showed cell death at a dilution ratio of 2. At a dilution ratio of 4 or 8, there was a marked decrease in cell viability. At a dilution ratio of 16, Subsequently, cell viability tests after photopolymerization were performed. Samples were irradiated with an LED-type light irradiator for 5 or 10 seconds, and cell viability was investigated using the three-dimensional culture method with collagen. Cell viability was evaluated using MTT assay. Cell viability in the 10-second irradiation group for all products was higher than in the 5-second irradiation group. Some products showed no significant difference in comparison with the negative control group. There was no relationship between the monomer composition or filler type of any product and cell viability. The results of this study showed that cell viability slightly differed among the 5 clinically available products. There was no product for which cell viability was particularly low.