Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
ORIGINAL ARTICLES
Effect of Acemannan on BMP-2 Expression in Primary Pulpal Fibroblasts And Periodontal Fibroblasts, in vitro Study
Nawaporn JITTAPIROMSAKSuwimon JETTANACHEAWCHANKITPeechanika LARDUNGDEEPolkit SANGVANICHPasutha Din THUNYAKITPISAL
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2007 年 4 巻 3 号 p. 149-154

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Periodontal fibroblasts and pulpal fibroblasts have been considered as the major cells responsible for new alveolar bone and dentin formation, respectively. Bone morphogenic protein-2 (BMP-2) has great clinical potential for inducing bone and dentin repair and regeneration. The objective of this study was to investigate the effect of acemannan, polysaccharide fraction of aloe vera gel on BMP-2 expression in primary human periodontal fibroblasts and pulpal fibroblasts. Cells were treated with increasing concentration of acemannan for 24 and 48 hours. The reverse transcriptase-PCR and ELISA assays were used to investigate the effects of acemannan on the levels of BMP-2 mRNA and protein expression, respectively. The results from RT-PCR and ELISA revealed that acemannan induced BMP-2 mRNA level and protein synthesis in both cells. The effective concentrations of acemannan were 0.25-1 mg/ml. Collectively, these results suggest that acemannan is a potent plant polysaccharide for stimulating BMP-2 expression in periodontal fibroblasts and pulpal fibroblasts.

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© 2007 by Japanese Association of Regenerative Dentistry
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