抄録
To examine the effect of fluoride on the survival of osteoblasts, media containing various concentrations of fluoride, 0〜5.0×10-2 mol/L, were prepared and mouse osteoblast MC3T3-E1 cells were cultured. At the same time, media containing 0〜5.0×10-2 mol/L of chloride as an indicator of pH were also adjusted. Although osteoblasts grew well at fluoride levels below 1.0×10-3 mol/L, osteoblast survival was dramatically inhibited over 5.0×10-3 mol/L of fluoride despite the pH of the medium scarcely decreasing when compared with the control pH 7.81, and osteoblasts showing favorable survival with 2.0×10-2 mol/L of chloride even at pH 6.81. Fluorospectrophotometric observation showed quite different features between control cells and at higher concentrations of fluoride. These results suggested that fluoride dramatically inhibits osteoblast growth at concentrations above 5.0×10-3 mol/L in the medium solution.
