Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
ISSN-L : 1348-9623
ORIGINAL ARTICLE
An Attempt to Cell Differentiation in Three-dimensional Culture System Using Non-feeder ES-D3 Cells and Feeder Layer Type ES cells
Koichi IMAIShoji TAKEDAAkito TANOUEKazuaki NAKAMURAKazuhiko SUESEFumio WATARIHiromasa TAKASHIMA
著者情報
ジャーナル フリー

2011 年 8 巻 3 号 p. 203-211

詳細
抄録

The embryonic stem cell test (EST) is an in vitro assay that has been developed to assess the embryotoxic potential of chemicals and biomaterials. An attempt for improvement in that ES cells used in the EST protocol are restricted to ES-D3 cells. If other kinds of ES cell become available, its experimental application will be further usefulness. We compared the incidence of pulsation between ES-D3 cells requiring no feeder cells for cultivation and EL M3 cells or ES-R1-EGFP B2/EGFP cells requiring feeder cells, to explore the experimental possibility of using ES cells requiring feeder cells.
As the present results with ES-D3 and EL M3 cells were similar to those obtained under the two-dimensional condition, these two kinds of cell are thought to be equally available under the present three-dimensional conditions. On the other hand, because ES-R1-EGFP B2/EGFP cells did not show any pulsation at all in the three-dimensional culture, other experimental conditions for the three-dimensional culture method need to be established with those cells. Besides, it was suggested that similar results could be obtained with EL M3 cells requiring feeder cells for cultivation compared to those with ES-D3 cells.

著者関連情報
© Japanese Association of Regenerative Dentistry
前の記事 次の記事
feedback
Top