Evaluation of a 3-D, Woven-fabric, Composite Scaffold Using Experimental Canine Models of Bone Defects in Mandibles

抄録

A woven-fabric scaffold, composed of biodegradable poly-L-lactic acid resin fibers and injected with marrow-derived mesenchymal cells mixed with platelet-rich plasma, was developed to evaluate the in vivo performance of a cell-scaffold graft material in experimental bone defects created in the dog mandible. Defects of regular shape and significant size were created at furcation sites and on the mesial aspects of lower premolar roots and then treated with graft material and subjected to histological analysis after 4 and 8 weeks of healing. In the experimental group, bone regeneration in defects on the tooth root mesial aspect occurred at 8 weeks, accompanied by the regeneration of periodontal tissues, such as cementum and periodontal ligaments. In contrast, delayed and immature healing was observed in the control group defects with no graft material. The findings here indicated that a three-dimensional, woven-fabric, composite scaffold combined with mesenchymal cells and platelet-rich plasma could accelerate the regeneration of alveolar bone and periodontal tissues.