Examination of an evaluation method for health effects of exposure to gas and particulate matter Under Air-Liquid Interface Culture conditions.

-Gene expression analysis of airway epithelial cells exposed to nitrogen dioxide-

Abstract

To examine the usefulness of our cell exposure system for the evaluation of inflammatory responses, we analyzed the expression of inflammation-related genes in airway epithelial cells exposed to nitrogen dioxide using the cell exposure system. As a result, the expression change of inflammation-related genes induced by nitrogen dioxide could be clearly observed in lung carcinoma cells (A549), but not in primary cells (MucilAirTM). This result showed the usefulness of the cell exposure system for the evaluation of inflammatory response, however, it is necessary to further study the effective use of primary cells.